Insecticidal Activity of Commercial Oil and Petroleum Ether Extract of Clove Buds (Syzygium aromaticum) on Some Stored Grain Pests

Boraie, Doaa M.; Al-Akhdar, Hala H.; Sadek, Mariam G.

doi:10.21608/ajesa.2014.163858

Insecticidal Activity of Commercial Oil and Petroleum Ether Extract of Clove Buds (Syzygium aromaticum) on Some Stored Grain Pests

Document Type : Original research articles

Authors

¹ ’Stored Products and Grains Department, Plant Protection Res. Institute, Agric. Res. Center. Dokki, Giza, Egypt

² Crops and Cotton Mites Department, Plant Protection Res. Institute, Agric. Res. Center. Dokki, Giza, Egypt

10.21608/ajesa.2014.163858

Abstract

This study aimed to evaluate the insecticidal activity of commercial clove oil and clove petroleum ether extract (Syzygium oromaticum) on the adults of rice weevil, Sitophilus oryzne (L.); cowpea beetle, Callosobruchus maculatus (F.); red flour beetle, Triboleum cristeneum (H.) ; the mould mite, Tyrophagus putrescentiae (Shrank) and Dermntophagoides farinae (Shrank) at 30±2°C and 65±5% R.H. Besides, studying the efficacy of clove petroleum ether extract to protect grains during storage and if it had any effect on seeds germination plus analyzing the chemical constituents for both extract and commercial oil through GC/MS technique to study the difference in main constituents. Results showed complete mortality (%) at the highest concentration with S.oryzae, C.maculatus and T.putrescentiae while T.cnstenetim and D. farinae showed high resistance for both oil and extract. Accumulative mortality of pests increased gradually with the increase of concentrations and exposure period. The reproduction of females of all experimental pests was completely inhibited at the highest concentration. T. castaneum was less susceptible to tested oils compared with S.oryzae and C.maculntus. The extract was more powerful than commercial oil especially with T.casteneum and D. farinae which are known to have high resistance. Clove extract give complete protection for grains for 10 weeks with low significant on germination (%). The main constituents of clove commercial oil were eugenol (50.50%), cinnamaldehyde (15.72 %) and linoleic acid (9.52%) and those of the clove extract were eugenol (37.43%) eugenol acetate (11.47%), caryophellene (10.44 %), linoleic acid (9.42%) and caryophellene oxide (8.58%).

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